Science
Basic Techniques in Molecular Biology
Author: Stefan Surzycki
Publisher: Springer Science & Business Media
ISBN: 9783642569685
Category: Science
Page: 434
View: 807
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Science
Molecular Biology Techniques
Author: Susan Carson
Publisher: Academic Press
ISBN: 9780123855442
Category: Science
Page: 227
View: 371
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "projectâ approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Science
Techniques in Molecular Biology
Author: J.M. Walker
Publisher: Springer Science & Business Media
ISBN: 9789401165631
Category: Science
Page: 335
View: 352
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Medical
Advances in Molecular Techniques
Author: Rakesh S. Sengar
Publisher: CRC Press
ISBN: 9781351021210
Category: Medical
Page: 436
View: 188
Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.
Biochemistry
Basic Techniques in Biochemistry and Molecular Biology
Author: R. K. Sharma
Publisher: I. K. International Pvt Ltd
ISBN: 9788190746267
Category: Biochemistry
Page: 278
View: 363
Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.Techniques for Molecular Biology
Author: D Tagu
Publisher: CRC Press
ISBN: 0367446391
Category:
Page: 230
View: 700
This book explains the theoretical principles of numerous techniques of genomic studies developed recently in laboratories. The book is designed for any reader who is curious to know the bases of different techniques of manipulation of nucleic acids.
Science
Wood Formation in Trees
Author: Nigel J Chaffey
Publisher: CRC Press
ISBN: 9781134485062
Category: Science
Page: 384
View: 488
Trees are a major component of the biosphere and have played an important part in the world's history and culture. With the modern challenges of global warming and dwindling fossil fuel reserves, trees, and in particular their wood, can provide solutions. Unfortunately, too little is known about the biology of these plants, due largely to a lack of
Science
Analytical Techniques in Biochemistry and Molecular Biology
Author: Rajan Katoch
Publisher: Springer Science & Business Media
ISBN: 1441997857
Category: Science
Page: 441
View: 400
Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.
Science
Techniques in Molecular Medicine
Author: Friedhelm Hildebrandt
Publisher: Springer
ISBN: 3642478085
Category: Science
Page: 387
View: 816
This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual
Bioengineering
Molecular Biology and Biotechnology
Author: M. V. Parakhia
Publisher: New India Publishing
ISBN: 9380235194
Category: Bioengineering
Page: 386
View: 975
The text is divided into 36 chapters followed by detailed glossary. Most of the required protocols have been included and the book caters to the need of subjects like food microbiology, textile microbiology, medical microbiology, and agriculture microbiology etc. This text is just a guide line to set the hand. In actual working you will be doing much more beyond this text and that will be going to make us wiser. We hope that this text will prove as a good partner for those who set their hands on microbial biotechnology.
Biochemistry
Laboratory Techniques in Biochemistry and Molecular Biology: Techniques of lipidology
Author:
Publisher:
ISBN: 0720442001
Category: Biochemistry
Page: 610
View: 418
Science
Molecular Biology Techniques
Author: Walt Ream
Publisher: Academic Press
ISBN: 9780080536828
Category: Science
Page: 234
View: 699
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter