Science

Molecular Biology Techniques

Molecular Biology Techniques

Author: Sue Carson

Publisher: Academic Press

ISBN: 0128180242

Category: Science

Page: 275

View: 434

Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology-or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Science

Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology

Author: Stefan Surzycki

Publisher: Springer

ISBN: 3540666788

Category: Science

Page: 434

View: 637

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Genetic engineering

Techniques in Molecular Biology: High performance liquid chromatography of proteins and nucleic acids

Techniques in Molecular Biology: High performance liquid chromatography of proteins and nucleic acids

Author: John M. Walker

Publisher:

ISBN: LCCN:83009419

Category: Genetic engineering

Page: 333

View: 360

Medical

Morphology Methods

Morphology Methods

Author: Ricardo V. Lloyd

Publisher: Springer Science & Business Media

ISBN: 9781592591909

Category: Medical

Page: 422

View: 443

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.
Science

Fundamentals and Techniques of Biophysics and Molecular Biology

Fundamentals and Techniques of Biophysics and Molecular Biology

Author: Pranav Kumar

Publisher: Pathfinder Publication unit of PAPL

ISBN: 9789380473154

Category: Science

Page: 115

View: 669

Fundamentals and Techniques of Biophysics and Molecular Biology textbook has the primary goal to teach students about theoretical principles and applications of the key biophysical and molecular methods used in biochemistry and molecular biology. A substantial theoretical basis has been covered to understand key experimental techniques such as Chromatography, Electrophoresis, Spectroscopy, Mass spectrometry, Centrifugation, Microscopy, Flow cytometry, Chromatin immunoprecipitation, Immunotechniques, FRET and FRAP, Polymerase chain reaction, Phage display, Yeast two-hybrid assay, DNA sequencing, Biosensors, CRISPR/Cas systems so that students can make appropriate choices and efficient use of techniques. The most significant feature of this book is its clear, up-to-date and accurate explanations of mechanisms, rather than the mere description of facts and events. This book is published by Pathfinder Publication, New Delhi, India.
Science

Principles and Techniques of Molecular Biology

Principles and Techniques of Molecular Biology

Author: Simon Pade

Publisher: Callisto Reference

ISBN: 1641165529

Category: Science

Page: 239

View: 787

The branch of biology that deals with the study of the molecular basis of biological activity between biomolecules in different systems of a cell is referred to as molecular biology. It includes the interactions between DNA, RNA, proteins and their biosynthesis along with the regulation of these interactions. Molecular biology also makes use of techniques and concepts from the fields of genetics and biochemistry. Some of the techniques used within this field are molecular cloning, polymerase chain reaction, gel electrophoresis and allele-specific oligonucleotide. There are numerous processes which are studied within molecular biology such as replication, transcription and cell function. This book is a compilation of chapters that discuss the most vital concepts in the field of molecular biology. While understanding the long-term perspectives of the topics, it makes an effort in highlighting their impact as a modern tool for the growth of the discipline. This textbook will provide comprehensive knowledge to the readers.
Technology & Engineering

Pattern Recognition in Computational Molecular Biology

Pattern Recognition in Computational Molecular Biology

Author: Mourad Elloumi

Publisher: John Wiley & Sons

ISBN: 9781119078852

Category: Technology & Engineering

Page: 656

View: 179

A comprehensive overview of high-performance pattern recognition techniques and approaches to Computational Molecular Biology This book surveys the developments of techniques and approaches on pattern recognition related to Computational Molecular Biology. Providing a broad coverage of the field, the authors cover fundamental and technical information on these techniques and approaches, as well as discussing their related problems. The text consists of twenty nine chapters, organized into seven parts: Pattern Recognition in Sequences, Pattern Recognition in Secondary Structures, Pattern Recognition in Tertiary Structures, Pattern Recognition in Quaternary Structures, Pattern Recognition in Microarrays, Pattern Recognition in Phylogenetic Trees, and Pattern Recognition in Biological Networks. Surveys the development of techniques and approaches on pattern recognition in biomolecular data Discusses pattern recognition in primary, secondary, tertiary and quaternary structures, as well as microarrays, phylogenetic trees and biological networks Includes case studies and examples to further illustrate the concepts discussed in the book Pattern Recognition in Computational Molecular Biology: Techniques and Approaches is a reference for practitioners and professional researches in Computer Science, Life Science, and Mathematics. This book also serves as a supplementary reading for graduate students and young researches interested in Computational Molecular Biology.
Medical

High-yield Cell and Molecular Biology

High-yield Cell and Molecular Biology

Author: Ronald W. Dudek

Publisher: Lippincott Williams & Wilkins

ISBN: 078176887X

Category: Medical

Page: 274

View: 724

This completely revised and updated review book consolidates the most important clinical issues that medical students need to know to be prepared for questions on USMLE Step 1. The book reviews key cell biology concepts needed to study molecular biology, and reviews the key concepts of molecular biology necessary for clinical medical practice, Flow charts provide a clear overview of molecular biology techniques and how they are applied in medicine. A chapter on understanding the research literature provides a solid background in molecular biology protocol so that students can understand the purpose and thinking behind published research articles.

A Handbook of Techniques in Biochemistry and Molecular Biology

A Handbook of Techniques in Biochemistry and Molecular Biology

Author:

Publisher:

ISBN: 9383828412

Category:

Page: 102

View: 428

Science

A History of Molecular Biology

A History of Molecular Biology

Author: Michel Morange

Publisher:

ISBN: 0674001699

Category: Science

Page: 336

View: 512

Every day it seems the media focus on yet another new development in biology--gene therapy, the human genome project, the creation of new varieties of animals and plants through genetic engineering. These possibilities have all emanated from molecular biology. A History of Molecular Biology is a complete but compact account for a general readership of the history of this revolution. Michel Morange, himself a molecular biologist, takes us from the turn-of-the-century convergence of molecular biology's two progenitors, genetics and biochemistry, to the perfection of gene splicing and cloning techniques in the 1980s. Drawing on the important work of American, English, and French historians of science, Morange describes the major discoveries--the double helix, messenger RNA, oncogenes, DNA polymerase--but also explains how and why these breakthroughs took place. The book is enlivened by mini-biographies of the founders of molecular biology: Delbrück, Watson and Crick, Monod and Jacob, Nirenberg. This ambitious history covers the story of the transformation of biology over the last one hundred years; the transformation of disciplines: biochemistry, genetics, embryology, and evolutionary biology; and, finally, the emergence of the biotechnology industry. An important contribution to the history of science, A History of Molecular Biology will also be valued by general readers for its clear explanations of the theory and practice of molecular biology today. Molecular biologists themselves will find Morange's historical perspective critical to an understanding of what is at stake in current biological research.
Science

Techniques for Molecular Biology

Techniques for Molecular Biology

Author: D Tagu

Publisher: Science Publishers

ISBN: 1578083613

Category: Science

Page: 244

View: 941

With the underpinning role of forage legumes in the nitrogen economy and animal productivity from temperate grasslands certain to expand in the future, particularly in regions where their potential has not yet been realized, it is essential that the wealth of information currently available is widely disseminated. This book serves the purpose with very detailed information on and illustrations of 35 selected forage legume species that will contribute to more efficient and viable grassland farming.
Science

Techniques in Molecular Biology

Techniques in Molecular Biology

Author: J.M. Walker

Publisher: Springer Science & Business Media

ISBN: 9789401165631

Category: Science

Page: 335

View: 389

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.